Use of a surface plasmon resonance method to investigate antibiotic and plasma protein interactions.

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Baneres-Roquet F, Gualtieri M, Villain-Guillot P, Pugniere M, Leonetti JP

Use of a surface plasmon resonance method to investigate antibiotic and plasma protein interactions.

Antimicrob Agents Chemother. 2009 Apr;53(4):1528-31. doi: 10.1128/AAC.00971-08. Epub 2009 Jan 21.

PubMed ID

19164148 [ View in PubMed

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Abstract

The pharmacologic effect of an antibiotic is directly related to its unbound concentration at the site of infection. Most commercial antibiotics have been selected in part for their low propensity to interact with serum proteins. These nonspecific interactions are classically evaluated by measuring the MIC in the presence of serum. As higher-throughput technologies tend to lose information, surface plasmon resonance (SPR) is emerging as an informative medium-throughput technology for hit validation. Here we show that SPR is a useful automatic tool for quantification of the interaction of model antibiotics with serum proteins and that it delivers precise real-time kinetic data on this critical parameter.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Cefotaxime	Serum albumin	Protein	Humans	Unknown	Not Available	Details

Drug Carriers

Drug	Carrier	Kind	Organism	Pharmacological Action	Actions
Rifampicin	alpha1-acid glycoprotein (Protein Group)	Protein group	Humans	Unknown	Ligand	Details
Rifampicin	Serum albumin	Protein	Humans	Unknown	Ligand	Details
Vancomycin	alpha1-acid glycoprotein (Protein Group)	Protein group	Humans	No	Substrate	Details
Vancomycin	Histamine H1 receptor	Protein	Mouse	No	Substrate	Details
Vancomycin	Serum albumin	Protein	Humans	No	Substrate	Details

Binding Properties

Drug	Target	Property	Measurement	pH	Temperature (°C)
Cefotaxime	Serum albumin	Kd (nM)	12000	N/A	N/A	Details
Vancomycin	Serum albumin	Kd (nM)	12000	N/A	N/A	Details