A map of 75 human ribosomal protein genes.
Article Details
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Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC
A map of 75 human ribosomal protein genes.
Genome Res. 1998 May;8(5):509-23.
- PubMed ID
- 9582194 [ View in PubMed]
- Abstract
We mapped 75 genes that collectively encode >90% of the proteins found in human ribosomes. Because localization of ribosomal protein genes (rp genes) is complicated by the existence of processed pseudogenes, multiple strategies were devised to identify PCR-detectable sequence-tagged sites (STSs) at introns. In some cases we exploited specific, pre-existing information about the intron/exon structure of a given human rp gene or its homolog in another vertebrate. When such information was unavailable, selection of PCR primer pairs was guided by general insights gleaned from analysis of all mammalian rp genes whose intron/exon structures have been published. For many genes, PCR amplification of introns was facilitated by use of YAC pool DNAs rather than total human genomic DNA as templates. We then assigned the rp gene STSs to individual human chromosomes by typing human-rodent hybrid cell lines. The genes were placed more precisely on the physical map of the human genome by typing of radiation hybrids or screening YAC libraries. Fifty-one previously unmapped rp genes were localized, and 24 previously reported rp gene localizations were confirmed, refined, or corrected. Though functionally related and coordinately expressed, the 75 mapped genes are widely dispersed: Both sex chromosomes and at least 20 of the 22 autosomes carry one or more rp genes. Chromosome 19, known to have a high gene density, contains an unusually large number of rp genes (12). This map provides a foundation for the study of the possible roles of ribosomal protein deficiencies in chromosomal and Mendelian disorders.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID 40S ribosomal protein SA P08865 Details 60S ribosomal protein L8 P62917 Details 60S ribosomal protein L37 P61927 Details 60S ribosomal protein L3 P39023 Details 60S ribosomal protein L11 P62913 Details 60S ribosomal protein L10 P27635 Details 40S ribosomal protein S19 P39019 Details 40S ribosomal protein S28 P62857 Details 40S ribosomal protein S5 P46782 Details 40S ribosomal protein S9 P46781 Details