Characterization of methotrexate transport and its drug interactions with human organic anion transporters.
Article Details
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Takeda M, Khamdang S, Narikawa S, Kimura H, Hosoyamada M, Cha SH, Sekine T, Endou H
Characterization of methotrexate transport and its drug interactions with human organic anion transporters.
J Pharmacol Exp Ther. 2002 Aug;302(2):666-71.
- PubMed ID
- 12130730 [ View in PubMed]
- Abstract
Life-threatening drug interactions are known to occur between methotrexate and nonsteroidal anti-inflammatory drugs (NSAIDs), probenecid, and penicillin G. The purpose of this study was to characterize methotrexate transport, as well as to determine the site and the mechanism of drug interactions in the proximal tubule. Mouse proximal tubule cells stably expressing basolateral human organic anion transporters (hOAT1 and hOAT3) and apical hOAT (hOAT4) were established. The K(m) values for hOAT1-, hOAT3-, and hOAT4-mediated methotrexate uptake were 553.8 microM, 21.1 microM, and 17.8 microM, respectively. NSAIDs (salicylate, ibuprofen, ketoprofen, phenylbutazone, piroxicam, and indomethacin), probenecid, and penicillin G dose dependently inhibited methotrexate uptake mediated by hOAT1, hOAT3, and hOAT4. Kinetic analysis of inhibitory effects of these drugs on hOAT3-mediated methotrexate uptake revealed that these inhibitions were competitive. The K(i) values for the effects of salicylate, phenylbutazone, indomethacin, and probenecid on hOAT3-mediated methotrexate uptake were comparable with therapeutically relevant plasma concentrations of unbound drugs. In addition, in the presence of human serum albumin, the K(i) values were comparable with therapeutically relevant total plasma concentrations of drugs. In conclusion, these results suggest that methotrexate is taken up via hOAT3 and hOAT1 at the basolateral side of the proximal tubule and effluxed or taken up at the apical side via hOAT4. In addition, hOAT1, hOAT3, and hOAT4 are the sites of drug interactions between methotrexate and NSAIDs, probenecid, and penicillin G. Furthermore, it was predicted that hOAT3 is the site of drug interactions between methotrexate and salicylate, phenylbutazone, indomethacin, and probenecid in vivo.
DrugBank Data that Cites this Article
- Drug Targets
Drug Target Kind Organism Pharmacological Action Actions Benzylpenicillin Solute carrier family 22 member 8 Protein Humans UnknownSubstrateInhibitorDetails Probenecid Solute carrier family 22 member 11 Protein Humans YesInhibitorDetails Probenecid Solute carrier family 22 member 6 Protein Humans YesInhibitorDetails Probenecid Solute carrier family 22 member 8 Protein Humans YesInhibitorDetails - Drug Transporters
- Binding Properties
Drug Target Property Measurement pH Temperature (°C) Benzylpenicillin Solute carrier family 22 member 8 Ki (nM) 97600 N/A N/A Details Ibuprofen Solute carrier family 22 member 8 Ki (nM) 1170000 N/A N/A Details Indomethacin Solute carrier family 22 member 8 Ki (nM) 5950 N/A N/A Details Ketoprofen Solute carrier family 22 member 8 Ki (nM) 1160000 N/A N/A Details Phenylbutazone Solute carrier family 22 member 8 Ki (nM) 34700 N/A N/A Details Piroxicam Solute carrier family 22 member 8 Ki (nM) 1200000 N/A N/A Details Probenecid Solute carrier family 22 member 8 Ki (nM) 29800 N/A N/A Details Salicylic acid Solute carrier family 22 member 8 Ki (nM) 1020000 N/A N/A Details