Enrichment of glycopeptides for glycan structure and attachment site identification.

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Nilsson J, Ruetschi U, Halim A, Hesse C, Carlsohn E, Brinkmalm G, Larson G

Enrichment of glycopeptides for glycan structure and attachment site identification.

Nat Methods. 2009 Nov;6(11):809-11. doi: 10.1038/nmeth.1392. Epub 2009 Oct 18.

PubMed ID
19838169 [ View in PubMed
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Abstract

We present a method to enrich for glycoproteins from proteomic samples. Sialylated glycoproteins were selectively periodate-oxidized, captured on hydrazide beads, trypsinized and released by acid hydrolysis of sialic acid glycosidic bonds. Mass spectrometric fragment analysis allowed identification of glycan structures, and additional fragmentation of deglycosylated ions yielded peptide sequence information, which allowed glycan attachment site and protein identification. We identified 36 N-linked and 44 O-linked glycosylation sites on glycoproteins from human cerebrospinal fluid.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
ProthrombinP00734Details
Antithrombin-IIIP01008Details
SerotransferrinP02787Details
Thyroxine-binding globulinP05543Details
Neuron-specific vesicular protein calcyonQ9NYX4Details
Alpha-1-acid glycoprotein 1P02763Details
Alpha-1-antitrypsinP01009Details
Kininogen-1P01042Details
Apolipoprotein EP02649Details
CeruloplasminP00450Details
Plasma protease C1 inhibitorP05155Details
Beta-2-glycoprotein 1P02749Details
Monocyte differentiation antigen CD14P08571Details
Alpha-1-acid glycoprotein 2P19652Details
Complement C4-AP0C0L4Details
Apolipoprotein DP05090Details
Ig alpha-1 chain C regionP01876Details
Apolipoprotein C-IIIP02656Details
AfaminP43652Details
Alpha-1B-glycoproteinP04217Details
Alpha-2-HS-glycoproteinP02765Details
Complement factor HP08603Details
Complement factor IP05156Details
ClusterinP10909Details
Leucine-rich alpha-2-glycoproteinP02750Details
Pigment epithelium-derived factorP36955Details
Amyloid-like protein 1P51693Details
Insulin-like growth factor-binding protein 6P24592Details
Prostaglandin-H2 D-isomeraseP41222Details
HemopexinP02790Details
HaptoglobinP00738Details