DrugBank: Hyaluronidase (DB00070)

targets (2) carriers (1)

Identification

Name

Hyaluronidase

Accession Number

DB00070 (BIOD00022, BTD00022)

Type

biotech

Groups

approved

Description

Highly purified sheep hyaluronidase for administration by injection into the vitreous of the eye.

Protein structure

Display: 3D Structure

Protein chemical formula

C₂₄₅₅H₃₇₇₅N₆₁₇O₇₀₄S₂₁

Protein average weight

53870.9000

Sequences

>DB00070 sequence
LNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDR
LGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWA
RNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHL
WGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAAT
LYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASG
IVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSS
DYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAV
DVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIVSILFLIISSVASL

FASTA

Synonyms

HYAL-1
hyaluronidase
Hyaluronidase 1 precursor
Hyaluronidase PH-20
Sperm adhesion molecule 1
Sperm surface protein PH-20

Brand names

Vitragan
Vitrase (Ista Pharma)

Brand name mixtures

Not Available

Categories

Anesthetic Adjuvants
Permeabilizing Agents

CAS number

488712-31-8

Taxonomy

Kingdom

Not Available

Classes

Not Available

Substructures

Not Available

Pharmacology

Indication

For increase of absorption and distribution of other injected drugs and for rehydration.

Pharmacodynamics

Hyaluronidase hydrolyzes hyaluronic acid and increase diffusion of injected drugs, thus facilitating their absorption. Hyaluronidase is used for enhancing absorption and distribution of other injected drugs.

Mechanism of action

Hyaluronidase is a spreading or diffusing substance. It increase the permeability of connective tissue through the hydrolysis of hyaluronic acid. Hyaluronidase hydrolyzes hyaluronic acid by splitting the glucosaminidic bond between C1 of the glucosamine moiety and C4 of glucuronic acid. This temporarily decreases the viscosity of the cellular cement and increases diffusion of injected fluids or of localized transudates or exudates, thus facilitating their absorption.

Absorption

Not Available

Volume of distribution

Not Available

Protein binding

Not Available

Metabolism

Route of elimination

Not Available

Half life

Not Available

Clearance

Not Available

Toxicity

Not Available

Affected organisms

Humans and other mammals

Pathways

Not Available

Pharmacoeconomics

Manufacturers

Amphastar pharmaceutical inc
Primapharm inc
Ista pharmaceuticals
Baxter healthcare corp anesthesia and critical care

Packagers

Akorn Inc.
Amphastar Pharmaceuticals
Bausch & Lomb Inc.
Baxter International Inc.
Cardinal Health
Halozyme Therapeutics Inc.
ISTA Pharmaceuticals
Oso Biopharmaceuticals Manufacturing LLC
Primapharm Inc.
Professional Co.

Dosage forms

Not Available

Prices

Unit description	Cost	Unit
Vitrasert 4.5 mg implant	19200.0 USD	implant
Vitrase 6200 unit vial	967.2 USD	vial
Hyaluronidase powder	900.0 USD	g
Hylenex 150 unit/ml vial	108.0 USD	ml

Patents

Not Available

Properties

State

liquid

Melting point

Not Available

Experimental Properties

Property	Value	Source
hydrophobicity	-0.117	PhysProp
isoelectric point	5.73	Various sources

References

Synthesis Reference

Not Available

General Reference

Csoka AB, Scherer SW, Stern R: Expression analysis of six paralogous human hyaluronidase genes clustered on chromosomes 3p21 and 7q31. Genomics. 1999 Sep 15;60(3):356-61. Pubmed

External Links

Resource	Link
UniProt	P38567
Genbank	L13781
PharmGKB	PA449893
Drug Product Database	2041278
RxList	http://www.rxlist.com/cgi/generic/hylenex.htm
Drugs.com	http://www.drugs.com/cdi/hyaluronidase.html
Wikipedia	http://en.wikipedia.org/wiki/Hyaluronidase

ATC Codes

B06AA03

AHFS Codes

Not Available

PDB Entries

2PE4

FDA label

show (42.5 KB)

MSDS

show (72.5 KB)

Interactions

Drug Interactions

Drug	Interaction

Food Interactions

Not Available

Targets
1. Hyaluronic acid Pharmacological action: yes Actions: other References: Nelson, D.L., & Cox, M.M. (2005). Lehninger principles of biochemistry (4 ^th ed). New York, NY: W.H. Freeman and Company. doi:10.1002/bmb.2005.494033010419 2. Transforming growth factor beta-1 Pharmacological action: unknown Actions: inhibitor Multifunctional protein that control proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGFB1 and essentially all of them have specific receptors for this protein. It regulates the actions of many other growth factors and determines a positive or negative direction of their effects. It plays an important role in bone remodelling. It is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts Organism class: human UniProt ID: P01137 Gene: TGFB1 Protein Sequence: FASTA Gene Sequence: FASTA SNPs: SNPJam Report References: Chang NS: Hyaluronidase activation of c-Jun N-terminal kinase is necessary for protection of L929 fibrosarcoma cells from staurosporine-mediated cell death. Biochem Biophys Res Commun. 2001 May 4;283(2):278-86. Pubmed Locci P, Marinucci L, Lilli C, Martinese D, Becchetti E: Transforming growth factor beta 1-hyaluronic acid interaction. Cell Tissue Res. 1995 Aug;281(2):317-24. Pubmed Chang NS: Hyaluronidase enhancement of TNF-mediated cell death is reversed by TGF-beta 1. Am J Physiol. 1997 Dec;273(6 Pt 1):C1987-94. Pubmed Chang NS: Transforming growth factor-beta protection of cancer cells against tumor necrosis factor cytotoxicity is counteracted by hyaluronidase (review). Int J Mol Med. 1998 Dec;2(6):653-9. Pubmed

Targets

1. Hyaluronic acid

Pharmacological action: yes
Actions: other

References:

Nelson, D.L., & Cox, M.M. (2005). Lehninger principles of biochemistry (4 ^th ed). New York, NY: W.H. Freeman and Company. doi:10.1002/bmb.2005.494033010419

2. Transforming growth factor beta-1

Pharmacological action: unknown
Actions: inhibitor

Multifunctional protein that control proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGFB1 and essentially all of them have specific receptors for this protein. It regulates the actions of many other growth factors and determines a positive or negative direction of their effects. It plays an important role in bone remodelling. It is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts

Organism class: human
UniProt ID: P01137 Link_out

Gene: TGFB1 Link_out

Protein Sequence: FASTA
Gene Sequence: FASTA
SNPs: SNPJam Report Link_out

References:

Chang NS: Hyaluronidase activation of c-Jun N-terminal kinase is necessary for protection of L929 fibrosarcoma cells from staurosporine-mediated cell death. Biochem Biophys Res Commun. 2001 May 4;283(2):278-86. Pubmed
Locci P, Marinucci L, Lilli C, Martinese D, Becchetti E: Transforming growth factor beta 1-hyaluronic acid interaction. Cell Tissue Res. 1995 Aug;281(2):317-24. Pubmed
Chang NS: Hyaluronidase enhancement of TNF-mediated cell death is reversed by TGF-beta 1. Am J Physiol. 1997 Dec;273(6 Pt 1):C1987-94. Pubmed
Chang NS: Transforming growth factor-beta protection of cancer cells against tumor necrosis factor cytotoxicity is counteracted by hyaluronidase (review). Int J Mol Med. 1998 Dec;2(6):653-9. Pubmed

Carriers
1. Serum albumin Actions: inhibitor Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood UniProt ID: P02768 Gene: ALB Protein Sequence: FASTA Gene Sequence: FASTA SNPs: SNPJam Report References: Maingonnat C, Victor R, Bertrand P, Courel MN, Maunoury R, Delpech B: Activation and inhibition of human cancer cell hyaluronidase by proteins. Anal Biochem. 1999 Mar 1;268(1):30-4. Pubmed Parameswaran S, Brown LV, Ibbott GS, Lai-Fook SJ: Effect of concentration and hyaluronidase on albumin diffusion across rabbit mesentery. Microcirculation. 1999 Jun;6(2):117-26. Pubmed

Carriers

1. Serum albumin

Actions: inhibitor

Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood

UniProt ID: P02768 Link_out

Gene: ALB

Protein Sequence: FASTA
Gene Sequence: FASTA
SNPs: SNPJam Report Link_out

References:

Maingonnat C, Victor R, Bertrand P, Courel MN, Maunoury R, Delpech B: Activation and inhibition of human cancer cell hyaluronidase by proteins. Anal Biochem. 1999 Mar 1;268(1):30-4. Pubmed
Parameswaran S, Brown LV, Ibbott GS, Lai-Fook SJ: Effect of concentration and hyaluronidase on albumin diffusion across rabbit mesentery. Microcirculation. 1999 Jun;6(2):117-26. Pubmed

Comments

Drug created on June 13, 2005 07:24 / Updated on August 04, 2011 21:36

This project is supported by Genome Alberta & Genome Canada, a not-for-profit organization that is leading Canada's national genomics strategy with $600 million in funding from the federal government. This project is also supported in part by GenomeQuest, Inc., an enterprise genomic information company serving the life science community.